J Plant Res. 2007 Jan;120(1):17-29. Epub 2007 Feb 2.
DNA banking for plant breeding, biotechnology and biodiversity evaluation.
Source
Department of Botany, School of Natural Sciences, University of Dublin, Trinity College, Dublin, Ireland. trevor.hodkinson@tcd.ie
Abstract
The manipulation of DNA is routine practice in botanical research and has made a huge impact on plant breeding, biotechnology and biodiversity evaluation. DNA is easy to extract from most plant tissues and can be stored for long periods in DNA banks. Curation methods are well developed for other botanical resources such as herbaria, seed banks and botanic gardens, but procedures for the establishment and maintenance of DNA banks have not been well documented. This paper reviews the curation of DNA banks for the characterisation and utilisation of biodiversity and provides guidelines for DNA bank management. It surveys existing DNA banks and outlines their operation. It includes a review of plant DNA collection, preservation, isolation, storage, database management and exchange procedures. We stress that DNA banks require full integration with existing collections such as botanic gardens, herbaria and seed banks, and information retrieval systems that link such facilities, bioinformatic resources and other DNA banks. They also require efficient and well-regulated sample exchange procedures. Only with appropriate curation will maximum utilisation of DNA collections be achieved.
Veja o link para os Bancos de DNA na Barra à direita do blog!
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